| Congresso Brasileiro de Microbiologia 2023 | Resumo: 727-2 | ||||
Resumo:Bacterial resistance has become a serious health problem worldwide. In this sense, multidrug-resistant Klebsiella pneumoniae poses a great threat to hospitalized patients, mainly those in intensive care units. This bacterium is associated with urinary tract and blood infections, sepsis and pneumonia. Among their mechanisms of virulence, the ability to form biofilm stands out, which make them inaccessible against immune system and antimicrobials action. In this context, benzoic acid (BA) is classified as a carboxylic acid, found naturally in plant and animal tissues, has been considered as a promising alternative therapy due to its antimicrobial potential. Therefore, the aim was to evaluate the antimicrobial activity of BA and its effect on biofilms formed by multidrug-resistant K. pneumoniae. For this, 25 K. pneumoniae isolates obtained from patients hospitalized at the University Hospital of the Federal University of Vale do São Francisco and a standard strain from the American Type Culture Collection (ATCC 13883) were used. These were removed from storage at -80 ºC, cultured to obtain fresh colonies and submitted to antimicrobial activity evaluation by the broth microdilution method to determine the minimum inhibitory concentration (MIC) and bactericidal concentration (MBC) of BA. Bacteria biofilm production and the interference test of 1/2 and 1/4 of the MIC of benzoic acid in the process of biofilm formation was carried out by the adherence to polyethylene microplates method, and staining with gentian violet. It was possible to observe a MIC of 2,000 µg/mL for all isolates and 4,000 µg/mL for ATCC 13883, while the MBC was 2,000 µg/mL for 50% of the isolates and 4,000 µg/mL for the remaining part. As for the ability to form biofilm, 96.15% (25) of samples were classified as weak and 3.85% (1) as strong biofilm producers. Bacteria previously classified as weak biofilm producers ceased producing them, while those considered strong producers showed a significant decrease in their capacity, becoming weak producers. With this, it is concluded that BA is capable of interfering in biofilm formation process by K. pneumoniae. Palavras-chave: ESKAPE, Infections, Public Health, Therapeutic Alternatives Agência de fomento:CAPES, CNPq, FACEPE, HU-UNIVASF/EBSERH | |||||